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memglow560  (Cytoskeleton Inc)


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    Structured Review

    Cytoskeleton Inc memglow560
    (A) Western blot analysis of MDA-MB-231 cell and EV lysates comparing common EV marker s , TSG101 and CD9, organelle marker Calnexin, and Actin loading control. Equal amounts of protein were loaded for cells and EVs. (B) Size distribution of isolated MDA-MB-231 EVs as determined by NTA. Data is represented as mean ± SD (grey). (C) dSTORM image from a representative experiment showing detection of CD9 (yellow) and 5-EU (magenta) in isolated MDA-MB-231 EVs after incubation of donor cells with 5-EU. Scale bar = 1µm. Bottom right: zoomed-in view of one cluster showing coincidence of CD9 and 5-EU. Scale bar = 200nm. (D) Quantitative cluster analysis of dSTORM data. Results are expressed as the percentage of 5-EU + clusters relative to <t>MemGlow560</t> + clusters and compared to EVs isolated from DMSO-treated donor cells.
    Memglow560, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/memglow560/bio_rxiv__2025__08__28__672797-90-6-7?v=Cytoskeleton+Inc
    Average 95 stars, based on 21 article reviews
    memglow560 - by Bioz Stars, 2026-07
    95/100 stars

    Images

    1) Product Images from "Visualizing extracellular vesicle-mediated RNA transfer using a novel metabolic labeling approach"

    Article Title: Visualizing extracellular vesicle-mediated RNA transfer using a novel metabolic labeling approach

    Journal: bioRxiv

    doi: 10.1101/2025.08.28.672797

    (A) Western blot analysis of MDA-MB-231 cell and EV lysates comparing common EV marker s , TSG101 and CD9, organelle marker Calnexin, and Actin loading control. Equal amounts of protein were loaded for cells and EVs. (B) Size distribution of isolated MDA-MB-231 EVs as determined by NTA. Data is represented as mean ± SD (grey). (C) dSTORM image from a representative experiment showing detection of CD9 (yellow) and 5-EU (magenta) in isolated MDA-MB-231 EVs after incubation of donor cells with 5-EU. Scale bar = 1µm. Bottom right: zoomed-in view of one cluster showing coincidence of CD9 and 5-EU. Scale bar = 200nm. (D) Quantitative cluster analysis of dSTORM data. Results are expressed as the percentage of 5-EU + clusters relative to MemGlow560 + clusters and compared to EVs isolated from DMSO-treated donor cells.
    Figure Legend Snippet: (A) Western blot analysis of MDA-MB-231 cell and EV lysates comparing common EV marker s , TSG101 and CD9, organelle marker Calnexin, and Actin loading control. Equal amounts of protein were loaded for cells and EVs. (B) Size distribution of isolated MDA-MB-231 EVs as determined by NTA. Data is represented as mean ± SD (grey). (C) dSTORM image from a representative experiment showing detection of CD9 (yellow) and 5-EU (magenta) in isolated MDA-MB-231 EVs after incubation of donor cells with 5-EU. Scale bar = 1µm. Bottom right: zoomed-in view of one cluster showing coincidence of CD9 and 5-EU. Scale bar = 200nm. (D) Quantitative cluster analysis of dSTORM data. Results are expressed as the percentage of 5-EU + clusters relative to MemGlow560 + clusters and compared to EVs isolated from DMSO-treated donor cells.

    Techniques Used: Western Blot, Marker, Control, Isolation, Incubation

    (A) Overexpression of UCK2 in donor cells enhances the 5-EU to 5-EU monophosphate conversion. As a result, more 5-EU monophosphate is converted to 5-EU triphosphate by UMP-CMPK and NDPK, and a higher ethynyl labeling density and more sensitive detection is obtained using click chemistry. Created in BioRender. Vader, P. (2025) https://BioRender.com/3pwgjvv Lentiviral DNA construct engineered for stable overexpression of UCK2 in donor cells. UCK2 is tagged with a FLAG-tag enabling detection of UCK2 expression. Additionally, an internal ribosomal entry site (IRES) is encoded upstream of a blasticidin (BSD) resistance gene for generating and maintaining stable expression of UCK2. (C) Western blot analysis for FLAG and GAPDH expression in MDA-MB-231 UCK2 + cell lysate and MDA-MB-231 wildtype cell lysate. Equal amounts of protein were loaded. (D) Confocal microscopy image of MDA-MB-231 UCK2 + donor cells. FLAG expression (green) was detected through immunofluorescence, 5-EU (magenta) through click chemistry, and nuclei (cyan) using Hoechst33342. (E) Fluorescence microscopy images showing a comparison of 5-EU labeling (magenta) in MDA-MB-231 UCK2 + and MDA-MB-231 wildtype donor cells upon incubation with 5-EU for 1 hour, 2 hours, 4 hours, and 20 hours. (F) Quantification of fluorescent signal from microscopy images in E. Mean fluorescence intensity per cell is represented over time for wildtype donor cells and UCK2+ donor cells. Data is presented as mean ± SD (G) Representative dSTORM image of EVs isolated from MDA-MB-231 UCK2 + after 5-EU treatment. EVs were labeled using CD9 antibodies (yellow) and RNA was labeled using 5-EU (magenta). (H) Quantitative cluster analysis of dSTORM experiment. Data are represented as percentage of 5-EU + clusters/MemGlow560 + clusters and compared to EVs isolated from DMSO treated UCK2 + donor cells.
    Figure Legend Snippet: (A) Overexpression of UCK2 in donor cells enhances the 5-EU to 5-EU monophosphate conversion. As a result, more 5-EU monophosphate is converted to 5-EU triphosphate by UMP-CMPK and NDPK, and a higher ethynyl labeling density and more sensitive detection is obtained using click chemistry. Created in BioRender. Vader, P. (2025) https://BioRender.com/3pwgjvv Lentiviral DNA construct engineered for stable overexpression of UCK2 in donor cells. UCK2 is tagged with a FLAG-tag enabling detection of UCK2 expression. Additionally, an internal ribosomal entry site (IRES) is encoded upstream of a blasticidin (BSD) resistance gene for generating and maintaining stable expression of UCK2. (C) Western blot analysis for FLAG and GAPDH expression in MDA-MB-231 UCK2 + cell lysate and MDA-MB-231 wildtype cell lysate. Equal amounts of protein were loaded. (D) Confocal microscopy image of MDA-MB-231 UCK2 + donor cells. FLAG expression (green) was detected through immunofluorescence, 5-EU (magenta) through click chemistry, and nuclei (cyan) using Hoechst33342. (E) Fluorescence microscopy images showing a comparison of 5-EU labeling (magenta) in MDA-MB-231 UCK2 + and MDA-MB-231 wildtype donor cells upon incubation with 5-EU for 1 hour, 2 hours, 4 hours, and 20 hours. (F) Quantification of fluorescent signal from microscopy images in E. Mean fluorescence intensity per cell is represented over time for wildtype donor cells and UCK2+ donor cells. Data is presented as mean ± SD (G) Representative dSTORM image of EVs isolated from MDA-MB-231 UCK2 + after 5-EU treatment. EVs were labeled using CD9 antibodies (yellow) and RNA was labeled using 5-EU (magenta). (H) Quantitative cluster analysis of dSTORM experiment. Data are represented as percentage of 5-EU + clusters/MemGlow560 + clusters and compared to EVs isolated from DMSO treated UCK2 + donor cells.

    Techniques Used: Over Expression, Labeling, Construct, FLAG-tag, Expressing, Western Blot, Confocal Microscopy, Immunofluorescence, Fluorescence, Microscopy, Comparison, Incubation, Isolation



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    memglow560  (Cytoskeleton Inc)
    95
    Cytoskeleton Inc memglow560
    (A) Western blot analysis of MDA-MB-231 cell and EV lysates comparing common EV marker s , TSG101 and CD9, organelle marker Calnexin, and Actin loading control. Equal amounts of protein were loaded for cells and EVs. (B) Size distribution of isolated MDA-MB-231 EVs as determined by NTA. Data is represented as mean ± SD (grey). (C) dSTORM image from a representative experiment showing detection of CD9 (yellow) and 5-EU (magenta) in isolated MDA-MB-231 EVs after incubation of donor cells with 5-EU. Scale bar = 1µm. Bottom right: zoomed-in view of one cluster showing coincidence of CD9 and 5-EU. Scale bar = 200nm. (D) Quantitative cluster analysis of dSTORM data. Results are expressed as the percentage of 5-EU + clusters relative to <t>MemGlow560</t> + clusters and compared to EVs isolated from DMSO-treated donor cells.
    Memglow560, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/memglow560/bio_rxiv__2025__08__28__672797-90-6-7?v=Cytoskeleton+Inc
    Average 95 stars, based on 1 article reviews
    memglow560 - by Bioz Stars, 2026-07
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Western blot analysis of MDA-MB-231 cell and EV lysates comparing common EV marker s , TSG101 and CD9, organelle marker Calnexin, and Actin loading control. Equal amounts of protein were loaded for cells and EVs. (B) Size distribution of isolated MDA-MB-231 EVs as determined by NTA. Data is represented as mean ± SD (grey). (C) dSTORM image from a representative experiment showing detection of CD9 (yellow) and 5-EU (magenta) in isolated MDA-MB-231 EVs after incubation of donor cells with 5-EU. Scale bar = 1µm. Bottom right: zoomed-in view of one cluster showing coincidence of CD9 and 5-EU. Scale bar = 200nm. (D) Quantitative cluster analysis of dSTORM data. Results are expressed as the percentage of 5-EU + clusters relative to MemGlow560 + clusters and compared to EVs isolated from DMSO-treated donor cells.

    Journal: bioRxiv

    Article Title: Visualizing extracellular vesicle-mediated RNA transfer using a novel metabolic labeling approach

    doi: 10.1101/2025.08.28.672797

    Figure Lengend Snippet: (A) Western blot analysis of MDA-MB-231 cell and EV lysates comparing common EV marker s , TSG101 and CD9, organelle marker Calnexin, and Actin loading control. Equal amounts of protein were loaded for cells and EVs. (B) Size distribution of isolated MDA-MB-231 EVs as determined by NTA. Data is represented as mean ± SD (grey). (C) dSTORM image from a representative experiment showing detection of CD9 (yellow) and 5-EU (magenta) in isolated MDA-MB-231 EVs after incubation of donor cells with 5-EU. Scale bar = 1µm. Bottom right: zoomed-in view of one cluster showing coincidence of CD9 and 5-EU. Scale bar = 200nm. (D) Quantitative cluster analysis of dSTORM data. Results are expressed as the percentage of 5-EU + clusters relative to MemGlow560 + clusters and compared to EVs isolated from DMSO-treated donor cells.

    Article Snippet: Isolated EVs were labeled with 200nM MemGlow560 (Cytoskeleton) for 30 minutes at RT.

    Techniques: Western Blot, Marker, Control, Isolation, Incubation

    (A) Overexpression of UCK2 in donor cells enhances the 5-EU to 5-EU monophosphate conversion. As a result, more 5-EU monophosphate is converted to 5-EU triphosphate by UMP-CMPK and NDPK, and a higher ethynyl labeling density and more sensitive detection is obtained using click chemistry. Created in BioRender. Vader, P. (2025) https://BioRender.com/3pwgjvv Lentiviral DNA construct engineered for stable overexpression of UCK2 in donor cells. UCK2 is tagged with a FLAG-tag enabling detection of UCK2 expression. Additionally, an internal ribosomal entry site (IRES) is encoded upstream of a blasticidin (BSD) resistance gene for generating and maintaining stable expression of UCK2. (C) Western blot analysis for FLAG and GAPDH expression in MDA-MB-231 UCK2 + cell lysate and MDA-MB-231 wildtype cell lysate. Equal amounts of protein were loaded. (D) Confocal microscopy image of MDA-MB-231 UCK2 + donor cells. FLAG expression (green) was detected through immunofluorescence, 5-EU (magenta) through click chemistry, and nuclei (cyan) using Hoechst33342. (E) Fluorescence microscopy images showing a comparison of 5-EU labeling (magenta) in MDA-MB-231 UCK2 + and MDA-MB-231 wildtype donor cells upon incubation with 5-EU for 1 hour, 2 hours, 4 hours, and 20 hours. (F) Quantification of fluorescent signal from microscopy images in E. Mean fluorescence intensity per cell is represented over time for wildtype donor cells and UCK2+ donor cells. Data is presented as mean ± SD (G) Representative dSTORM image of EVs isolated from MDA-MB-231 UCK2 + after 5-EU treatment. EVs were labeled using CD9 antibodies (yellow) and RNA was labeled using 5-EU (magenta). (H) Quantitative cluster analysis of dSTORM experiment. Data are represented as percentage of 5-EU + clusters/MemGlow560 + clusters and compared to EVs isolated from DMSO treated UCK2 + donor cells.

    Journal: bioRxiv

    Article Title: Visualizing extracellular vesicle-mediated RNA transfer using a novel metabolic labeling approach

    doi: 10.1101/2025.08.28.672797

    Figure Lengend Snippet: (A) Overexpression of UCK2 in donor cells enhances the 5-EU to 5-EU monophosphate conversion. As a result, more 5-EU monophosphate is converted to 5-EU triphosphate by UMP-CMPK and NDPK, and a higher ethynyl labeling density and more sensitive detection is obtained using click chemistry. Created in BioRender. Vader, P. (2025) https://BioRender.com/3pwgjvv Lentiviral DNA construct engineered for stable overexpression of UCK2 in donor cells. UCK2 is tagged with a FLAG-tag enabling detection of UCK2 expression. Additionally, an internal ribosomal entry site (IRES) is encoded upstream of a blasticidin (BSD) resistance gene for generating and maintaining stable expression of UCK2. (C) Western blot analysis for FLAG and GAPDH expression in MDA-MB-231 UCK2 + cell lysate and MDA-MB-231 wildtype cell lysate. Equal amounts of protein were loaded. (D) Confocal microscopy image of MDA-MB-231 UCK2 + donor cells. FLAG expression (green) was detected through immunofluorescence, 5-EU (magenta) through click chemistry, and nuclei (cyan) using Hoechst33342. (E) Fluorescence microscopy images showing a comparison of 5-EU labeling (magenta) in MDA-MB-231 UCK2 + and MDA-MB-231 wildtype donor cells upon incubation with 5-EU for 1 hour, 2 hours, 4 hours, and 20 hours. (F) Quantification of fluorescent signal from microscopy images in E. Mean fluorescence intensity per cell is represented over time for wildtype donor cells and UCK2+ donor cells. Data is presented as mean ± SD (G) Representative dSTORM image of EVs isolated from MDA-MB-231 UCK2 + after 5-EU treatment. EVs were labeled using CD9 antibodies (yellow) and RNA was labeled using 5-EU (magenta). (H) Quantitative cluster analysis of dSTORM experiment. Data are represented as percentage of 5-EU + clusters/MemGlow560 + clusters and compared to EVs isolated from DMSO treated UCK2 + donor cells.

    Article Snippet: Isolated EVs were labeled with 200nM MemGlow560 (Cytoskeleton) for 30 minutes at RT.

    Techniques: Over Expression, Labeling, Construct, FLAG-tag, Expressing, Western Blot, Confocal Microscopy, Immunofluorescence, Fluorescence, Microscopy, Comparison, Incubation, Isolation